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ISRCTN
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ISRCTN81682521
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DOI
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10.1186/ISRCTN81682521
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ClinicalTrials.gov identifier
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EudraCT number
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Public title
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Role of TLR2 in the Sensing of oxidants and ensuing Inflammation
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Scientific title
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Role of TLR2 in the Sensing of oxidants and ensuing Inflammation: Implications for therapeutic Intervention - an observational study
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Acronym
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TSI
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Serial number at source
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5843
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Study hypothesis
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The aim of this project is to study how oxidants are sensed by Toll like receptors (TLRs)
1. Determine the effects that oxidants have on the ectodomain of TLR2
2. Assess the requirements for the TLR adaptor proteins MyD88 and TIRAP in oxidants dependant signalling
3. To assess the involvement of other PRRs in oxidant induced inflammation
4. Assess the differences in gene activation between classical TLR2 ligands and oxidants
5. Grow out blood-derived stem cells for assessment
More details can be found at: http://public.ukcrn.org.uk/Search/StudyDetail.aspx?StudyID=5843
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Lay summary
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Lay summary under review 3
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Ethics approval
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Royal Brompton & Harefield NHS Trust Ethics Committee, 08/H0708/69
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Study design
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Observational study
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Countries of recruitment
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United Kingdom
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Disease/condition/study domain
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Immunology and inflammation
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Participants - inclusion criteria
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Healthy volunteer (either a smoker or non-smoker)
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Participants - exclusion criteria
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Does not meet inclusion criteria
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Anticipated start date
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06/08/2008
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Anticipated end date
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08/08/2013
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Status of trial
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Ongoing |
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Patient information material
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Not available in web format, please use the contact details below to request a patient information sheet
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Target number of participants
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Planned Sample Size: 100
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Interventions
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Blood will be take form volunteers who are either healthy smokers (within 20 mins of smoking a cigarette) or non-smokers. The blood will either be directly plated out into 96 well plates or cells isolated or grown out and characterised for their responses to various agonists and a number of mediators and cytokines will be measured. The total time a volunteer is need for is 20 mins although they may be asked back to participate again.
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Primary outcome measure(s)
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The blood or cellular responses will be compared between smokers and non-smokers. To assess the differences that oxidative exposure will have on smokers just 20 mins after having a cigarette.
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Secondary outcome measure(s)
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No secondary outcome measures
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Sources of funding
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Wellcome Trust (UK) ref: WT083429MA
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Trial website
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Publications
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Contact name
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Dr
Mark
Paul-Clark
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Address
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Royal Brompton & Harefield NHS Foundation Trust
Sydney Street
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City/town
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London
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Zip/Postcode
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SW3 6NP
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Country
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United Kingdom
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Email
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m.paul-clark@imperial.ac.uk
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Sponsor
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Wellcome Trust (UK)
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Address
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Queen Square
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City/town
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London
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Zip/Postcode
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WC1N 3BG
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Country
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United Kingdom
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Date applied
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25/10/2012
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Last edited
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26/02/2013
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Date ISRCTN assigned
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26/02/2013
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